Journal: Cell Communication and Signaling : CCS

Article Title: JC124 confers multimodal neuroprotection in epilepsy by suppressing NLRP3 inflammasome activation: evidence from animal and human neuronal models

doi: 10.1186/s12964-025-02239-3

Figure Lengend Snippet: JC124 treatment suppresses seizures in KA-induced epileptic mice. A Study design timeline. B Structure of JC124. C Latency to the first convulsive SRS and ( D ) the number of convulsive SRSs ( n = 10). E Representative images of LFP recordings in the post-SE + vehicle, post-SE + JC124, and post-SE + VPA groups. F The number of SLEs, ( G ) the mean duration of SLE, and ( H ) the cumulative duration of SLEs in the hippocampal LFP ( n = 8). KA-induced epileptic mice treated with JC124 exhibited a marked increase in latency to the first convulsive SRS, accompanied by a reduction in the number of convulsive SRSs, as well as a decrease in the number and cumulative duration of SLEs. Data are represented as mean ± S.E.M. *** p < 0.001. ns indicates no statistical significance

Article Snippet: In accordance with previous studies [ , ] and our preliminary experiments, JC124 (CSNpharm, IL, USA; 50 mg/kg, intraperitoneally) or VPA (TargetMol, MA, USA; 200 mg/kg, intraperitoneally) was administered once daily for 28 days.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Histone Deacetylase Inhibitors Reduce the Number of Herpes Simplex Virus-1 Genomes Initiating Expression in Individual Cells

doi: 10.3389/fmicb.2016.01970

Figure Lengend Snippet: List of inhibitors used in this work.

Article Snippet: Valproic Acid (VPA) , Merck , 4 mM.

Techniques: Concentration Assay

Journal: Frontiers in Microbiology

Article Title: Histone Deacetylase Inhibitors Reduce the Number of Herpes Simplex Virus-1 Genomes Initiating Expression in Individual Cells

doi: 10.3389/fmicb.2016.01970

Figure Lengend Snippet: Effect of histone modifying enzyme inhibitors on the number of viral genomes expressed per cell. Vero cells were infected at multiplicity of infection (MOI) 100, 50, or 10 with a mixture of the three fluorophore expressing herpes simplex virus 1 (HSV-1) strains. (A–D) Representative images taken 6–8 hpi of infected cells at MOI 100 (A,B) or 10 (C,D) show the variability in the hues as a result of the three different fluorescent colors (mCherry – red, YFP – Green, and mTurq2 – blue). Infection was carried out in the presence (B,D) or absence (A,C) of Trichostatin A (TSA). Scale bars 50 μM. (E) Images were used for calculating the average number of genomes expressed per cell. The infected cells were incubated with different inhibitors, 3 h prior to infection. Color coded as follows: No inhibitor – red, TSA – lightest blue, Suberohydroxamic Acid (SBX) – light blue, Valproic Acid (VPA) – blue, Suberoylanilide Hydroxamic Acid (SAHA) – dark blue, and Anacardic Acid (AA) in gray. Each bar represents at least two biological repeats and error bars represent standard deviations between the repeats. ∗ P < 0.05, ∗∗ P < 0.01; ∗∗∗ P < 0.001; by t -test.

Article Snippet: Valproic Acid (VPA) , Merck , 4 mM.

Techniques: Infection, Expressing, Incubation